| Approved Methods for Microbiological Testing |
|
| APHA Decimal Dilution |
Approved |
Total Coliforms and Fecal Coliforms - G.A. waters and Shellfish |
Growing Area Classification |
Recommended Procedures, APHA 1970 |
Microbiology |
| 12 tube Single Dilution |
Approved |
Total Coliforms and Fecal Coliforms - G.A. waters (cannot be used with Systematic Random Sampling) and Shellfish (end product depurated shellfish only) |
Growing Area Classification and Depuration |
Redman 1974, Proceedings of 8th National Shellfish Sanitation Workshop (J. Springer) NSSP Interpretation 17-IV-@.02-102 |
Microbiology |
| Other APHA (10 tube, 10 ml portions) |
Approved |
Total Coliforms - UV Treated Process water |
Wet Storage and Depuration |
Standard Methods, 18th edition, 1992 Section 9221 |
Microbiology |
| mEndo agar LES |
Approved |
Total Coliforms - UV Treated Process water |
Wet Storage and Depuration |
Standard Methods, 20th edition, 1998. Section 9222 B. ; Proposal 11-111 |
Microbiology |
| A-1M Decimal Dilution MPN |
Approved |
Fecal Coliforms - G.A. waters |
Growing Area Classification |
Method 978.23, AOAC 1990 |
Microbiology |
| A-1M 12 tube Single Dilution MPN |
Approved |
Fecal Coliforms - G.A. waters (cannot be used with Systematic Random Sampling) |
Growing Area Classification |
Method 978.23, AOAC 1990; NSSP Interpretation 17-III-@.02-100 |
Microbiology |
| A-1M without salicin |
Approved |
Fecal Coliforms - G.A. waters (requires comparability testing) |
Growing Area Classification |
Karolus et. al 2003 |
Microbiology |
| mTEC |
Approved |
Fecal Coliforms - G.A. waters |
Growing Area Classification |
Rippey et. al 1987 (grandfathered) |
Microbiology
|
| ETCP |
Approved |
Fecal Coliforms - Shellfish (Clams only) |
Depuration |
Cabelli and Heffernan 1970 |
Microbiology |
| Standard Plate Count |
Approved |
Total Bacteria - Shellfish |
Shellfish transportation and handling |
Recommended Procedures, APHA 1970 |
Microbiology |
| Approved Limited Use Methods for Microbiological Testing |
|
| Modified Double Agar Overlay (MSC) |
Approved Limited Use |
MSC - Shellfish (Hard and Soft Clams and Oysters) |
Tracking tool for point-sources; est. buffer zones around WWTP; reopen after WWTP discharge; assess before/after relay |
SLV (2009; Proposal 09-114 [soft shelled clams and oyster];13-120 [hard clams]) |
Microbiology |
| Approved Methods for Vibrio Enumeration |
|
| EIA |
Approved |
Vibrio vulnificus (Vv) - Shellfish |
Antibody no longer available |
Reference no longer available (FDA BAM 1992) |
Under Development |
| Vibrio MPN ( with PCR confirmation) |
Approved |
Vibrio parahaemolyticus (Vp) - Shellfish |
PHP |
FDA BAM Ch9 |
Under Development |
| Direct Plating Method (probe) |
Approved |
Vibrio parahaemolyticus (Vp) (tlh & tdh) - Shellfish |
Growing Area Reopening |
FDA BAM Ch 9; Cook et. al 1999 |
Checklist Under Interim Approval (17-110) |
| Vibrio MPN (with biochem or probe confirmation) |
Approved |
Vibrio vulnificus (Vv) - Shellfish |
PHP |
FDA BAM Ch9 & SLVs & Wright et. al 1993 |
Under Development |
| Vibrio MPN (with biochem or probe confirmation) |
Approved |
Vibrio parahaemolyticus (Vp) - Shellfish |
PHP |
FDA BAM Ch9 & SLVs & McCarthy et. al 1999 |
Under Development |
| SYBR Green 1 QPCR-MPN |
Approved |
Vibrio vulnificus (Vv) - Oysters |
PHP |
SLV (2009; Proposal 09-103) |
Under Development |
| MPN- Real Time PCR |
Approved |
Vibrio parahaemolyticus (Vp) (tdh & trh) - Oysters |
PHP and/or Growing Area Reopening |
SLV (2015; Proposal 15-111); Kinsey et al. 2015 |
PCR Microbiology |
| MPN- Real Time PCR |
Approved |
Vibrio parahaemolyticus (Vp) (tlh) - Oysters |
PHP and/or Growing Area Reopening |
SLV (2015; Proposal 15-113); Kinsey et al. 2015 |
PCR Microbiology |
| MPN - Real Time PCR |
Approved |
Vibrio vulnificus (Vv) - Oysters |
PHP |
SLV (2019; Proposal 19-126); Kinsey et al. 2015 |
Under Development |
| Approved Methods for Marine Biotoxin Testing |
|
| APHA Mouse Bioassay (MBA) |
Approved |
PSP and NSP - Shellfish |
Growing Area Management |
Recommended Procedures, APHA 1970 |
MBA/Scotia/PSP, MBA/NSP |
| Receptor Binding Assay (RBA) |
Approved |
PSP - Shellfish (Mussels only) |
Growing Area Management |
SLV (2013; Proposal 13-114) |
Checklist Under Review (19-141) |
| PCOX |
Approved |
PSP - Shellfish (mussels, clams, oysters, scallops) |
Growing Area Management |
Proposal 09-104; Rourke et. al 2008; Proposal 15-108 |
PSP/PCOX |
| LC MS/MS |
Approved |
DSP - clams only |
Growing Area Management |
SLV (2017; Proposal 17-103) |
Checklist under Interim Approval(19-136) |
| Domoic Acid HPLC |
Approved |
ASP (Domoic Acid) - Shellfish |
Growing Area Management |
Quilliam et. al 1991 |
Domoic/HPLC |
| Approved Limited Use Methods for Marine Biotoxin Testing |
|
| Abraxis Shipboard ELISA |
Approved Limited Use |
PSP - Shellfish (surf clams and quahogs) |
Growing Area Management |
SLV 05-111 (extraction) & 09-107 (ELISA) |
Under Development |
| Scotia Rapid Test |
Approved Limited Use |
PSP - Shellfish screen |
Growing Area Management |
Proposal 03-116 and 05-110 |
MBA/Scotia/PSP |
| Reveal 2.0 ASP |
Approved Limited Use |
ASP (Domoic Acid) - Shellfish screen |
Growing Area Management |
SLV (2013; Proposal 13-112) |
Under Development |
| Receptor Binding Assay (RBA) |
Approved Limited Use |
PSP - Shellfish screening for clams and scallops only |
Growing Area Management |
SLV (2013; Proposal 13-114) |
Checklist Under Review (19-141) |
| ELISA |
Approved Limited Use |
NSP - oysters, hard clams, sunray venus clams; positive tests require MBA |
Growing Area Management |
SLV (2017; Proposal 17-107 ) |
Marbionc |